高效毛细管电泳法测定两种不同颜色拳参饮片中没食子酸含量

高杨亚雅, 朱尘琪, 郭爽, 张萍, 邱百灵, 吴迪, 梁启慧, 高也, 韩南银

中国药学杂志 ›› 2016, Vol. 51 ›› Issue (23) : 2063-2067.

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中国药学杂志 ›› 2016, Vol. 51 ›› Issue (23) : 2063-2067. DOI: 10.11669/cpj.2016.23.018
论著

高效毛细管电泳法测定两种不同颜色拳参饮片中没食子酸含量

  • 高杨亚雅1, 朱尘琪1, 郭爽1, 张萍2, 邱百灵1, 吴迪1, 梁启慧1, 高也1, 韩南银1*
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Determination of Gallic Acid in Quanshen Pieces of Two Different Colors by HPCE

  • GAOYANG Ya-ya1, ZHU Chen-qi1, GUO Shuang1, ZHANG Ping2, QIU Bai-ling1, WU Di1, LIANG Qi-hui1, GAO Ye1, HAN Nan-yin1*
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摘要

目的 建立毛细管电泳测定拳参中没食子酸的方法。检测紫红色和棕红色两种商品拳参饮片中没食子酸的含量是否有差异。方法 采用高效毛细管电泳法,缓冲液为pH 9.0的20 mmol·L-1硼砂缓冲溶液;运行电压为20 kV;检测波长为290 nm;进样压力5 kPa,进样时间10 s;运行温度为室温。结果 整个分析过程在6 min内完成。湖北、山东、河北3 个产地7 个批次的拳参饮片中,紫红色拳参饮片没食子酸的含量: 湖北2.70%,山东12.08%、7.79%,河北2.01%、5.78%、6.97%、4.22%; 棕红色拳参饮片中没食子酸的含量: 湖北1.35%,山东1.81%、2.94%,河北1.87%、3.42%、0.44%、1.52%。结论 该方法简单,经济,快速,可以为拳参质量控制提供依据。同时,紫红色拳参饮片中没食子酸的含量高于棕红色饮片。

Abstract

OBJECTIVE To establish a high-performance capillary electrophoresis (CE) method for determinating gallic acid in commercial Quanshen pieces of two different colors,amaranth and brownish red. METHODS CE-UV method was used in the following condition: BGE 20 mmol·L-1 sodium borate solution, voltage 20 kV, detection wavelength 290 nm, sample injection 10 s at 5 kPa, capillary temperature room temperature. RESULTS A quantitative method was successfully developed to determine gallic acid in Quanshen pieces within 6 min. The contents of gallic acid in 7 batches of amaranth colored Quanshen pieces were 2.70% (Hubei province), 12.08% and 7.79% (Shandong province), 2.01%, 5.78%, 6.97%, and 4.22% (Hebei province), respectively. The contents of gallic acid in brownish red colored Quanshen pieces were 1.35% (Hubei province), 1.81% and 3.42% (Shandong province), 1.87%, 3.42%, 0.44%, and 1.52% (Hebei province), respectively. CONCLUSION The method is rapid, economical and simple and can provide a powerful quality control measure for Quanshen pieces. The contents of gallic acid in the amaranth colored Quanshen pieces are higher than those in the brownish red colored pieces.

关键词

拳参 / 紫红色拳参饮片 / 棕红色拳参饮片 / 没食子酸 / 高效毛细管电泳法

Key words

Quanshen / amaranth colored Quanshen piece / brownish red colored Quanshen piece / gallic acid / HPCE

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高杨亚雅, 朱尘琪, 郭爽, 张萍, 邱百灵, 吴迪, 梁启慧, 高也, 韩南银. 高效毛细管电泳法测定两种不同颜色拳参饮片中没食子酸含量[J]. 中国药学杂志, 2016, 51(23): 2063-2067 https://doi.org/10.11669/cpj.2016.23.018
GAOYANG Ya-ya, ZHU Chen-qi, GUO Shuang, ZHANG Ping, QIU Bai-ling, WU Di, LIANG Qi-hui, GAO Ye, HAN Nan-yin. Determination of Gallic Acid in Quanshen Pieces of Two Different Colors by HPCE[J]. Chinese Pharmaceutical Journal, 2016, 51(23): 2063-2067 https://doi.org/10.11669/cpj.2016.23.018
中图分类号: R917   

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